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Master thesis

Développement d’une technique de siRNA chez l’amibe Acanthamoeba castellanii

Abstract : Free-living amoeba are organisms capable of changing from a vegetative form, the trophozoite, to a dormant form, the cyst, under stressful environmental conditions. The signalling pathways of this process remain poorly understood. RNA interference is a genetic tool that would allow to modulate the expression of genes in the amoeba model Acanthamoeba castellanii, as it has already been performed in previous studies. Modulation of theses genes expressions would allow the study of their function. The objective of this internship was to adapt a siRNA protocol using cellulose synthase as the target gene, for which it was shown that expression increases during the encystation process. Although the first step, internalization of siRNA in the cell, was observed, cellulose synthase expression was not downregulated. Several critical points related to this genetic tool remain to be optimized
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Master thesis
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https://hal.univ-lorraine.fr/hal-03359628
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Submitted on : Thursday, September 30, 2021 - 12:35:52 PM
Last modification on : Saturday, April 30, 2022 - 4:03:13 PM
Long-term archiving on: : Friday, December 31, 2021 - 7:57:28 PM

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  • HAL Id : hal-03359628, version 1

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Amélie Jessu. Développement d’une technique de siRNA chez l’amibe Acanthamoeba castellanii. Microbiologie et Parasitologie. 2020. ⟨hal-03359628⟩

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