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Master thesis

Optimisation d’une méthode de détection de transfert de gène

Abstract : The progressive increase of multidrug resistance around the world has been recognized as a public health priority. Horizontal gene transfers (HGT) are part of the main causes of the spread of antibiotic resistance genes. This is why it is necessary to understand the mechanisms of transfer within complex communities in order to find counter-measures to limit them. The objective of this study is to optimize an in vivo murine model for studying the transfer of the plasmid pB10 within the intestinal microbiota. This model uses two approaches for the detection and the quantification of the plasmid and its bacterial host: qPCR and flow cytometry. The impact of the matrix (type of sample, mode of preservation, plasmid-donor's inoculum) on the quantification by qPCR must first be studied. Optimization also involves adjusting the flow cytometry protocol using strains harboring different fluorescent labels. Once optimized, this model will open up several leads for studying factors influencing gene transfers in vivo.
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Master thesis
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https://hal.univ-lorraine.fr/hal-03360223
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Submitted on : Thursday, September 30, 2021 - 4:27:17 PM
Last modification on : Saturday, April 30, 2022 - 4:02:56 PM

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  • HAL Id : hal-03360223, version 1

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Bernadette Grondin. Optimisation d’une méthode de détection de transfert de gène. Microbiologie et Parasitologie. 2020. ⟨hal-03360223⟩

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