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A hybrid electrochemical flow reactor to couple H 2 oxidation to NADH regeneration for biochemical reactions

Abstract : Beta-nicotinamide adenine dinucleotide (NAD + /NADH) is an important enzymatic co-factor that can be efficiently regenerated using a rhodium-based catalyst as electron transfer mediator (i.e. [Cp*Rh(bpy)Cl] + , where Cp* = pentamethylcyclopentadienyl and bpy = 2,2-bipyridine). Here, the above mediated regeneration of NADH is implemented in a redox flow bioreactor hybridized with a gas diffusion electrode for hydrogen oxidation. The reactor was initially optimized with respect to rhodium complex and NAD + concentrations, humidification of the hydrogen gas, flow rates of both H 2 gas and electrolytic solution, and solution pH. The integration of an enzymatic reaction consuming the generated NADH was then investigated in a flow process, combining in series the electrochemical reactor to a biochemical cell with immobilized L-lactic dehydrogenase for the conversion of pyruvate to lactate. A high activity was achieved with a turnover number up to 370 h-1 for NADH regeneration. Coupled electrochemical regeneration to enzymatic reaction led to total turnover number values of 2000 and 6.3*10 6 for NADH electrochemical regeneration and bioconversion, respectively.
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https://hal.univ-lorraine.fr/hal-03425413
Contributor : Francois Lapicque Connect in order to contact the contributor
Submitted on : Wednesday, November 10, 2021 - 5:52:18 PM
Last modification on : Wednesday, October 5, 2022 - 3:59:51 AM
Long-term archiving on: : Friday, February 11, 2022 - 7:48:09 PM

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Wassim El Housseini, François Lapicque, Alain Walcarius, Mathieu Etienne. A hybrid electrochemical flow reactor to couple H 2 oxidation to NADH regeneration for biochemical reactions. Electrochemical Science Advances, 2022, 2, pp.e202100012. ⟨10.1002/elsa.202100012⟩. ⟨hal-03425413⟩

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