Genomic Signatures of a Major Adaptive Event in the Pathogenic Fungus Melampsora larici-populina - Université de Lorraine Access content directly
Journal Articles Genome Biology and Evolution Year : 2022

Genomic Signatures of a Major Adaptive Event in the Pathogenic Fungus Melampsora larici-populina

Antoine Persoons
Clémentine Louet
Pascal Frey

Abstract

The recent availability of genome-wide sequencing techniques has allowed systematic screening for molecular signatures of adaptation, including in nonmodel organisms. Host–pathogen interactions constitute good models due to the strong selective pressures that they entail. We focused on an adaptive event which affected the poplar rust fungus Melampsora larici-populina when it overcame a resistance gene borne by its host, cultivated poplar. Based on 76 virulent and avirulent isolates framing narrowly the estimated date of the adaptive event, we examined the molecular signatures of selection. Using an array of genome scan methods based on different features of nucleotide diversity, we detected a single locus exhibiting a consistent pattern suggestive of a selective sweep in virulent individuals (excess of differentiation between virulent and avirulent samples, linkage disequilibrium, genotype–phenotype statistical association, and long-range haplotypes). Our study pinpoints a single gene and further a single amino acid replacement which may have allowed the adaptive event. Although our samples are nearly contemporary to the selective sweep, it does not seem to have affected genome diversity further than the immediate vicinity of the causal locus, which can be explained by a soft selective sweep (where selection acts on standing variation) and by the impact of recombination in mitigating the impact of selection. Therefore, it seems that properties of the life cycle of M. larici-populina, which entails both high genetic diversity and outbreeding, has facilitated its adaptation.
Fichier principal
Vignette du fichier
AGAP_2021_SEGURA_GENOME_BIOL_EVOL_CC_BY_NC_Genomic_signatures_of_major_adpative_event_pathogenic_fungus_Melampsora_Iarici_populina.pdf (1016.62 Ko) Télécharger le fichier
Origin Publisher files allowed on an open archive

Dates and versions

hal-03606205 , version 1 (17-05-2022)

Licence

Identifiers

Cite

Antoine Persoons, Agathe Maupetit, Clémentine Louet, Axelle Andrieux, Anna Lipzen, et al.. Genomic Signatures of a Major Adaptive Event in the Pathogenic Fungus Melampsora larici-populina. Genome Biology and Evolution, 2022, 14 (1), 14 p. ⟨10.1093/gbe/evab279⟩. ⟨hal-03606205⟩
106 View
70 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More