Ribosomal RNA 2′- O -methylations regulate translation by impacting ribosome dynamics - Université de Lorraine Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2022

Ribosomal RNA 2′- O -methylations regulate translation by impacting ribosome dynamics

Abstract

Significance The presence of RNA chemical modifications has long been known, but their precise molecular consequences remain unknown. 2′- O -methylation is an abundant modification that exists in RNA in all domains of life. Ribosomal RNA (rRNA) represents a functionally important RNA that is heavily modified by 2′- O -methylations. Although abundant at functionally important regions of the rRNA, the contribution of 2′- O -methylations to ribosome activities is unknown. By establishing a method to disturb rRNA 2′- O -methylation patterns, we show that rRNA 2′- O -methylations affect the function and fidelity of the ribosome and change the balance between different ribosome conformational states. Our work links 2′- O -methylation to ribosome dynamics and defines a set of critical rRNA 2′- O -methylations required for ribosome biogenesis and others that are dispensable.

Dates and versions

hal-03612834 , version 1 (18-03-2022)

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Cite

Sohail Khoshnevis, R. Elizabeth Dreggors-Walker, Virginie Marchand, Yuri Motorin, Homa Ghalei. Ribosomal RNA 2′- O -methylations regulate translation by impacting ribosome dynamics. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (12), pp.e2117334119. ⟨10.1073/pnas.2117334119⟩. ⟨hal-03612834⟩
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