Epimutations in both the TESK2 and MMACHC promoters in the Epi-cblC inherited disorder of intracellular metabolism of vitamin B12 - Université de Lorraine
Journal Articles Clinical Epigenetics Year : 2022

Epimutations in both the TESK2 and MMACHC promoters in the Epi-cblC inherited disorder of intracellular metabolism of vitamin B12

Wendy Chung

Abstract

Background epi- cblC is a recently discovered inherited disorder of intracellular vitamin B 12 metabolism associating hematological, neurological, and cardiometabolic outcomes. It is produced by an epimutation at the promoter common to CCDC163P and MMACHC , which results from an aberrant antisense transcription due to splicing mutations in the antisense PRDX1 gene neighboring MMACHC . We studied whether the aberrant transcription produced a second epimutation by encompassing the CpG island of the TESK2 gene neighboring CCDC163P . Methods We unraveled the methylome architecture of the CCDC163P – MMACHC CpG island (CpG:33) and the TESK2 CpG island (CpG:51) of 17 epi- cblC cases. We performed an integrative analysis of the DNA methylome profiling, transcriptome reconstruction of RNA-sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-Seq) of histone H3, and transcription expression of MMACHC and TESK2 . Results The PRDX1 splice mutations and activation of numerous cryptic splice sites produced antisense readthrough transcripts encompassing the bidirectional MMACHC / CCDC163P promoter and the TESK2 promoter, resulting in the silencing of both the MMACHC and TESK2 genes through the deposition of SETD2-dependent H3K36me3 marks and the generation of epimutations in the CpG islands of the two promoters. Conclusions The antisense readthrough transcription of the mutated PRDX1 produces an epigenetic silencing of MMACHC and TESK2 . We propose using the term 'epi-digenism' to define this epigenetic disorder that affects two genes. Epi- cblC is an entity that differs from cblC. Indeed, the PRDX1 and TESK2 altered expressions are observed in epi- cblC but not in cblC , suggesting further evaluating the potential consequences on cancer risk and spermatogenesis.
Fichier principal
Vignette du fichier
13148_2022_Article_1271.pdf (1.74 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive

Dates and versions

hal-03664165 , version 1 (23-01-2023)

Licence

Identifiers

Cite

Abderrahim Oussalah, Youssef Siblini, Sébastien Hergalant, Céline Chéry, Pierre Rouyer, et al.. Epimutations in both the TESK2 and MMACHC promoters in the Epi-cblC inherited disorder of intracellular metabolism of vitamin B12. Clinical Epigenetics, 2022, 14 (1), pp.52. ⟨10.1186/s13148-022-01271-1⟩. ⟨hal-03664165⟩
134 View
44 Download

Altmetric

Share

More