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Article Dans Une Revue Current Research in Food Science Année : 2022

Altering almond protein function through partial enzymatic hydrolysis for creating gel structures in acidic environment

Résumé

Protein inadequacy is the major problem for most plant-based dairy yoghurt substitutes. This study investigated three limited degree of hydrolysis (DH: 1%, 5%, and 9%) of almond protein and the combined effect of DH and hydrolysed almond protein (HP) to non-hydrolysed almond protein (NP) ratios (HP/NP: 40:60, 20:80, 10:90 and 5:95) on the physicochemical properties of resulting fermentation induced almond-based gel (yoghurt). The gel microstructure, particle size, firmness, pH, water holding capacity (WHC), lubrication, flow, and gelation characteristics were measured and associated with the DH, composition, and SDS-PAGE results. The results show significant differences in gel samples with the same HP/NP (40:60) ratio of protein but different protein DH. A higher DH (9%) resulted in samples with lower hardness (6.03 g), viscosity (0.11 Pa s at 50 s-1), cohesiveness (0.63) and higher friction (0.203 at 10 mm/s) compared to sample with 1% DH with higher hardness - 7.34 g, viscosity at 50 s−1 - 0.16 Pa s, cohesiveness - 0.86 and friction at 10 mm/s - 0.194. Comparing samples with the same DH (5%) but different HP/NP ratios showed smaller coarse microgel particles (21.36 μm) and lower hardness (7.17 g), viscosity (0.14 Pa s at 50 s−1) and friction value (0.189 at 10 mm/s) in samples with high HP/NP (40:60) compared to sample with low HP/NP (5:95) that contained significantly large coarse microgel particles (34.61 μm) with the gel being very hard (9.38 g), highly viscous (0.32 Pa s at 50 s−1), and less lubricating (0.220 at 10 mm/s).

Dates et versions

hal-03704730 , version 1 (25-06-2022)

Identifiants

Citer

Jia Zhao, Bhesh Bhandari, Claire Gaiani, Sangeeta Prakash. Altering almond protein function through partial enzymatic hydrolysis for creating gel structures in acidic environment. Current Research in Food Science, 2022, 5, pp.653-664. ⟨10.1016/j.crfs.2022.03.012⟩. ⟨hal-03704730⟩
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