A Novel Near‐IR Absorbing Ruthenium(II) Complex as Photosensitizer for Photodynamic Therapy and its Cetuximab Bioconjugates - Université de Lorraine Access content directly
Journal Articles ChemBioChem Year : 2023

A Novel Near‐IR Absorbing Ruthenium(II) Complex as Photosensitizer for Photodynamic Therapy and its Cetuximab Bioconjugates

Abstract

A novel Ru(II) cyclometalated photosensitizer (PS), Ru‐NH 2 , for photodynamic therapy (PDT) of formula [Ru(appy)(bphen) 2 ]PF 6 (where appy=4‐amino‐2‐phenylpyridine and bphen=bathophenanthroline) and its cetuximab (CTX) bioconjugates, Ru‐Mal‐CTX and Ru‐BAA‐CTX (where Mal=maleimide and BAA=benzoylacrylic acid) were synthesised and characterised. The photophysical properties of Ru‐NH 2 revealed absorption maxima around 580 nm with an absorption up to 725 nm. The generation of singlet oxygen ( 1 O 2 ) upon light irradiation was confirmed with a 1 O 2 quantum yield of 0.19 in acetonitrile. Preliminary in vitro experiments revealed the Ru‐NH 2 was nontoxic in the dark in CT‐26 and SQ20B cell lines but showed outstanding phototoxicity when irradiated, reaching interesting phototoxicity indexes (PI) >370 at 670 nm, and >150 at 740 nm for CT‐26 cells and >50 with NIR light in SQ20B cells. The antibody CTX was successfully attached to the complexes in view of the selective delivery of the PS to cancer cells. Up to four ruthenium fragments were anchored to the antibody (Ab), as confirmed by MALDI‐TOF mass spectrometry. Nonetheless, the bioconjugates were not as photoactive as the Ru‐NH 2 complex.
Fichier principal
Vignette du fichier
A Novel Near-IR Absorbing Ruthenium(II) Complex.pdf (754.45 Ko) Télécharger le fichier
Origin Files produced by the author(s)
Licence

Dates and versions

hal-04235726 , version 1 (18-12-2023)

Licence

Identifiers

Cite

Marta Martínez-Alonso, Albert Gandioso, Chloé Thibaudeau, Xue Qin, Philippe Arnoux, et al.. A Novel Near‐IR Absorbing Ruthenium(II) Complex as Photosensitizer for Photodynamic Therapy and its Cetuximab Bioconjugates. ChemBioChem, 2023, 24 (15), ⟨10.1002/cbic.202300203⟩. ⟨hal-04235726⟩
56 View
50 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More