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Contribution à l'étude des déterminants génétiques impliqués dans le processus infectieux de Melampsora larici-populina, l'agent de la rouille foliaire du peuplier

Abstract : The leaf rust disease caused by Melampsora larici-populina (Mlp) is the main disease affecting poplar plantations in Europe with severe economic losses. The recent sequencing of the genome of Mlp (strain 98AG31) opens new perspectives to identify key genes involved in the fungal infection process and particularly those encoding fungal effectors that could manipulate host cell structure and function to facilitate host colonization. Analysis of the rust transcriptome during time course infection of poplar leaves, based on NimbleGen oligoarrays and massive EST sequencing led to the identification of genes related to fungal germination, biotrophy and sporulation. A consistant induction of genes encoding small-secreted proteins (SSPs) was observed during the biotrophic growth at 96 hours post-inoculation (hpi) but also at 168 hpi in the palisade mesophyll using laser capture microdissection. Mlp Secretome analysis, based on annotation, evolution and expression of genes encoding SSPs helped in identifying candidate poplar rust effectors. Some, specifically expressed in planta or showing homologies with known rust effectors were localized around the haustorium. Interestingly, other candidate genes, belonging to multigenic families under diversifying selection are cystein-rich, specifically expressed in planta and harbour a translocation signal potentially involved in effector export inside host cell. This genome-wide analysis of putative fungal effectors will contribute to the general knowledge of rust biology and will help to set new approaches to prevent and control the disease
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https://hal.univ-lorraine.fr/tel-01746338
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  • HAL Id : tel-01746338, version 1
  • PRODINRA : 179210

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Stéphane Hacquard. Contribution à l'étude des déterminants génétiques impliqués dans le processus infectieux de Melampsora larici-populina, l'agent de la rouille foliaire du peuplier. Sylviculture, foresterie. Université Henri Poincaré - Nancy 1, 2010. Français. ⟨NNT : 2010NAN10112⟩. ⟨tel-01746338⟩

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