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Caractérisation des déterminants géniques impliqués dans l'interaction entre un agent phytopathogène, Melampsora larici-populina, et le Peuplier

Abstract : Melampsora larici-populina (Mlp), the basidiomycete responsible for poplar rust, causes the most important damages in european poplar plantations. Nowadays, no more resistant poplar cultivars are available. In order to characterize defense mechanisms of poplar against Mlp, NBS-LRR R genes in the poplar genome were examined. We also carried out analysis of gene expression profils during qualitative and quantitative poplar resistance upon rust infection using cDNA micro and macro-arrays. In addition, a cDNA SSH library was constructed in order to identify genes specifically expressed during the incompatible interaction between poplar and rust. Up to 400 putative R genes were identified in the poplar genome, and some of them are expressed upon rust infection. Fungus growth was limited 24 h-pi and tissues near infection sites were lignified after 48 h-pi during the incompatible interaction of poplar and Mlp, corresponding to a qualitative resistance. Mlp develope large or small urediniosore 8 d-pi according to poplar genotype. A set of gene with a significantly regulated expression was identified using arrays and SSH cDNA library. Many of them correspond to plant disease gene and are regulated during qualitative and quantitative poplar resistance upon rust infection. However, these genes are expressed with lower intensity and later during the quantitative resistance than during the qualitative resistance.
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http://docnum.univ-lorraine.fr/prive/SCD_T_2007_0011_RINALDI.pdf
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https://hal.univ-lorraine.fr/tel-01746533
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Submitted on : Thursday, March 29, 2018 - 10:39:50 AM
Last modification on : Friday, February 5, 2021 - 3:43:29 AM

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  • HAL Id : tel-01746533, version 1

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Cecile Rinaldi. Caractérisation des déterminants géniques impliqués dans l'interaction entre un agent phytopathogène, Melampsora larici-populina, et le Peuplier. Sylviculture, foresterie. Université Henri Poincaré - Nancy 1, 2007. Français. ⟨NNT : 2007NAN10011⟩. ⟨tel-01746533⟩

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