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Etude structurale et fonctionnelle de motifs en "K-turn" présents dans les ARN, rôle dans l'assemblage et le mécanisme d'action de particules ribonucléoprotéiques impliquées dans la maturation des ARN

Abstract : In eukarya, the nucleolar U3 snoRNP plays a crucial role in 18S rRNA maturation. U3 snoRNA contains 2 domains (5' and 3'). We studied the role for 18S rRNA production of base-pair interactions formed between its 5' domain and the pre-rRNA. U3 snoRNA binds several proteins. Its 3' domain contains two protein anchoring sites (the C'/D and B/C motifs). They adopt a peculiar K-turn structure and each K-turn binds protein Snu13. Binding of this protein is required for recruitment of proteins Nop1, Nop56 and Nop58 on the C'/D motif and of protein Rrp9 on the B/C motif. We determined the RNA determinants, required for the specific recruitment of these proteins, in particular Rrp9p. The RNA binding domain of protein Snu13p has strong homologies with those of the archaeal L7Ae and vertebrate SBP2 proteins. We compared the RNA binding specificity of these 3 proteins. Finally, we studied RNA conformational changes in pre-ribosomal RNAs during the maturation process.
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http://docnum.univ-lorraine.fr/prive/SCD_T_2006_0108_CLERY.pdf
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https://hal.univ-lorraine.fr/tel-01746551
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Submitted on : Thursday, March 29, 2018 - 10:40:08 AM
Last modification on : Friday, February 26, 2021 - 3:02:02 PM

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  • HAL Id : tel-01746551, version 1

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Antoine Clery. Etude structurale et fonctionnelle de motifs en "K-turn" présents dans les ARN, rôle dans l'assemblage et le mécanisme d'action de particules ribonucléoprotéiques impliquées dans la maturation des ARN. Biochimie, Biologie Moléculaire. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN10108⟩. ⟨tel-01746551⟩

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