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Rôle de la mobilisation calcique dans la régulation de la réponse oxydative dans un modèle de neutrophiles humains

Abstract : In response to a stimulus, the oxidative response, resulting of the NADPH oxidase assembly, is a fundamental biological function of phagocytic cells. An excessive response is associated to chronical inflammations. In this study, we have investigated the relation between the elevation of cytosolic-free calcium concentration ([Ca2+]i) and oxydative response, induced by N-formyl-L-Methionyl-L-Leucyl-L-Phenylalanine (fMLF), in a model of neutrophils: differentiated HL-60 cells. We have demonstrated, by simultaneously measurements of [Ca2+]i and H2O2 production, that extracellular Ca2+ entry, regulated by the intracellular Ca2+ release stores, is necessary to the biological response initiation in response to fMLF. Cyclic ADP-ribose is implicated in this calcium mobilization. Furthermore, interleukin-8 priming of cells increases H2O2 production dependently of extracellular Ca2+ entry. By biochemical studies, we have estasblished that the translocation of Rac proteins is tightly correlated to an [Ca2+]i elevation, whereas with molecular studies we have identified capacitative channels probably implicated in the extracellular Ca2+ entry. The oxydative response, via Rac translocation is dependent on extracellular Ca2+ entry by the capacitative mechanism resulting of the calcium channels activation (potentiel transient receptors) stimulated by differents second messengers mobilizing Ca2+.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2006_0003_BRECHARD.pdf
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https://hal.univ-lorraine.fr/tel-01746647
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Submitted on : Thursday, March 29, 2018 - 10:41:51 AM
Last modification on : Tuesday, May 26, 2020 - 3:51:59 PM

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  • HAL Id : tel-01746647, version 1

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Sabrina Bréchard. Rôle de la mobilisation calcique dans la régulation de la réponse oxydative dans un modèle de neutrophiles humains. Biochimie, Biologie Moléculaire. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN10003⟩. ⟨tel-01746647⟩

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