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Détection et caractérisation des bactéries intracellulaires associées au champignon ectomycorhizien Laccaria bicolor S238N

Abstract : Laccaria bicolor S238N is an ectomycorrhizal fungus used commercially in France for controlled mycorrhization. During the production of the inoculum in fermentor, bacterial contaminations occur recurrently. Fluorescent in situ hybridization associated with confocal microscopy has allowed to detect several types of intracellular bacteria in the pure cultures of the fungus. Most of these endobacteria belong to the Firmicutes. Some have been identified as Paenibacilli, and belong to the same species as bacteria isolated from a L. bicolor S238N culture in fermentor. In contrast, in non axenic mycelium samples such as mycorrhizas, endobacteria affiliated to the a-proteobacteria were detected. Since some observations suggest that the endobacteria sometimes colonize live fungal cells, it could be a case of bacterial endosymbiosis.
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https://hal.univ-lorraine.fr/tel-01746762
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Submitted on : Thursday, March 29, 2018 - 10:44:23 AM
Last modification on : Wednesday, September 8, 2021 - 4:02:07 PM

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Joanne Bertaux. Détection et caractérisation des bactéries intracellulaires associées au champignon ectomycorhizien Laccaria bicolor S238N. Sylviculture, foresterie. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10165⟩. ⟨tel-01746762⟩

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