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Theses

Studio strutturale di proteine implicate in meccanismi di ossidoriduzione. Caratterizzazione di complessi 0 di intermedi di reazioni

Abstract : This PhD thesis concerned structural studies of non phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans (GAPN) and of Glutaredoxin (Grx) from Poplar Thrichorpora. GAPN belongs to the family of the aldehyde dehydrogenases (ALDH), enzymes which catalyse the oxidation of a broad range of aldehydes into their corresponding carboxylic acids. Their catalytic mechanism proceeds via two steps : acylation and deacylation. With the aim to characterize the structural and molecular factors associated to this reaction, we have solved the x-ray structure of the intermediate thioacylenzyme and the structure of the active site mutant T229S. Grx belongs to the thiol-disulphide oxydo-reductase family and is involved in the adaptative response during oxidative stress. It catalyses the reduction of disulfides bridges of different substrates. Since only few structural and biochemical information on GRX isolated from plants are actually available, crystallographic studies of Grx complexed with glutathione have been carried out to obtain more information on this class of enzymes.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2004_0126_D_AMBROSIO.pdf
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https://hal.univ-lorraine.fr/tel-01746774
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 10:44:39 AM
Last modification on : Thursday, February 25, 2021 - 10:00:02 AM

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Katia d'Ambrosio. Studio strutturale di proteine implicate in meccanismi di ossidoriduzione. Caratterizzazione di complessi 0 di intermedi di reazioni. Other. Université Henri Poincaré - Nancy 1, 2004. Italian. ⟨NNT : 2004NAN10126⟩. ⟨tel-01746774⟩

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