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Etudes sur le mécanisme d'action du petit ARN nucléolaire U3 de la levure Saccharomyces cerevisiae et sur la protéine Snu13p/15,5 kD associée à cet ARN et son homologue d'archaea L7Ae

Abstract : In eukarya, U3 snoRNA plays a crucial role in ribosomal RNA maturation. Its 5' domain forms several base-pair interactions with the pre-ribosomal RNA and its 3' domain contains two anchoring sites for the U3 snoRNP proteins, including Snu13p. We showed, in yeast, that the segments of the U3 snoRNA 5' domain, which are involved in the intermolecular base-pair interactions and the sequences linking these segments, have differential functional roles in the maturation process. Our structural, phylogenetic, and functional study of Snu13p binding on U3 snoRNA demonstrated the presence of two binding sites that form peculiar K-turn structure. In vivo experiments showed that mutations in the B/C motif are more deleterious for U3 snoRNA function than mutations in the C'/D motif. We compared the RNA binding specificity of Snu13p and its archaeal homolog L7Ae. Finally, we found a possible link between protein Rsa1, involved in the late steps of 60S subunit maturation, and U3 snoRNA.
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http://docnum.univ-lorraine.fr/prive/SCD_T_2003_0178_MARMIER_GOURRIER.pdf
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https://hal.univ-lorraine.fr/tel-01746840
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Submitted on : Thursday, March 29, 2018 - 10:45:54 AM
Last modification on : Tuesday, September 29, 2020 - 11:18:54 AM

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  • HAL Id : tel-01746840, version 1

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Nathalie Marmier-Gourrier. Etudes sur le mécanisme d'action du petit ARN nucléolaire U3 de la levure Saccharomyces cerevisiae et sur la protéine Snu13p/15,5 kD associée à cet ARN et son homologue d'archaea L7Ae. Biochimie, Biologie Moléculaire. Université Henri Poincaré - Nancy 1, 2003. Français. ⟨NNT : 2003NAN10178⟩. ⟨tel-01746840⟩

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