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Etudes morphologiques et fonctionnelles de l'estomac foetal humain xénogreffé chez la souris alymphocytaire T

Abstract : It's ethically unthinkable to study morphological and functional developments of the human stomach in utero. In contrast, the method developed by Pr DUPREZ and Dr ANGIOI, allows such studies. Human foetal stomachs 6-10 week-old from requested legal abortion were microsurgically engrafted in recipient nude and SCID mice. All grafts grew normally, and their size never exceed 3 x 4 x 4 cm whatever the graft delay. Peptic type gastric fistulae uses to develop after 90 graft-days. The development of stomach grafts was studied by using histological, histophysiological, ultrastructural and biochimieal methods and showed that by 60 graft-days glandular and muscular layers were mature as an adult type gastric mucosa. Host cells in stomach xenografts were restricted to some host fibromyoblasts in the lamina propria and to vascular endothelial cells which augmented with the graft duration. ,Parietal cells (H+ /K+ -A TPase and intrinsic factor positives), chief cells (pepsinogen positive), ECL, D and G cells were all fully differentiated. Stomach grafts secreted an acidic gastric juice, containing mucous, Na+, K+ and crions, intrinsic factor, pepsins and gastrin. Acid secretion was increased by pentagastrin and lowered by omeprazole, with modifications of subcellular structures of parietal cells, showed by transmission electron microscopy analysis.Transplants could be infected pennanently by Helicobacter pylori during the three-months of observation. All this results demonstrate that in vivo development of the human foetal stomach xenografts was normal but early than in utero. The acceleration of stomach differentiation but without changing the chronology is probably induced by host epigenetic factors.
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Submitted on : Thursday, March 29, 2018 - 10:47:02 AM
Last modification on : Wednesday, January 6, 2021 - 3:01:38 PM

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  • HAL Id : tel-01746891, version 1



Filipe Muhale. Etudes morphologiques et fonctionnelles de l'estomac foetal humain xénogreffé chez la souris alymphocytaire T. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2000. Français. ⟨NNT : 2000NAN10300⟩. ⟨tel-01746891⟩



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