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Contribution à l'étude de l'affinité des anticorps monoclonaux par des méthodes d'optique physique

Abstract : The mechanism of interaction involved in cell-cell adhesion has significant implications in biological and medical sciences. Particularly, the erythrocyte interaction and erythrocyte adhesion to the vascular tissue have been widely studied. The purpose of this work was to study the cell-cell interaction, by the quantification of the intercellular binding energy by two reo-optical techniques. In the present study, red blood cell agglutinates induced by monocional antibodies have been used as an experimental model system of specific cell-cell interaction. First, the energy involved in intercellular dissociation was quantified by a microscopie approach (flow channel). By this method a uniform shear stress parallel to the two-ceUs agglutinate interface is applied. The agglutinates, subjected to the shear stress, were observed under the microscope and the images were recorded. The analysis of these images allowed the direct quantification of the degree of dissociation in response to applied shear flow. Second, the energy involved in immunological agglutination was studied by a global approach (erythroaggregometer). Backscattered light intensity from the agglutinate suspension was evaluated. These agglutinates were dissociated by imposing a constant shear stress in a transparent Couette viscometer. ln both techniques, a direct correlation between the surface density of monoclonal antibody molecules on the erythrocyte membrane and the different parameters was found. ln conclusion, the energy involved in cell-cell dissociation has been assessed by experimental measurements and theoretical analysis. The approaches used in this erythrocyte-antibody system may be extended, with proper modifications, to other cell systems involving other interacting molecules.
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Laura Verónica Pla. Contribution à l'étude de l'affinité des anticorps monoclonaux par des méthodes d'optique physique. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2000. Français. ⟨NNT : 2000NAN10302⟩. ⟨tel-01746894⟩

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