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Etude des modalités de mort cellulaire suite à un traitement photodynamique avec le Foscan® in vitro. Relation entre la distribution intratumorale du Foscan® et l'efficacité de la thérapie photodynamique in vivo chez la souris

Abstract : Photodynamic therapy (PDT) is a treatment modality for small confined light-accessible tumors. PDT requires the combined action of a photosensitizer, light and oxygen. Tumor eradication occurs directly through damage to tumor cells and indirectly through damage to the vessels and by immune system induction. The possibility of modulating cell death modalities by fluence rate was investigated on spheroids subjected to Foscan® based-PDT. The results showed that for a given moderate level of photocytotoxicity (LD6s), the use of a low fluence rate of irradiation could enhance the percentage of cell death mediated by apoptosis. The relation between Foscan® based-PDT photocytotoxicity and photosensitizer accumulation in tumor, plasma and leukocyte was examined on tumor-bearing mice. The greatest efficiency was evidenced in animaIs irradiated 6h and 12h after Foscan® injection, in correlation with best photosensitizer accumulation in leukocytes, suggesting a large contribution of indirect effects. This hypothesis was refined by a study of Foscan® intratumor distribution by confocal microscopy. The images suggest that both indirect and direct damage are required to get the best treatment photocytotoxicity.
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http://docnum.univ-lorraine.fr/prive/SCD_T_2004_0249_MAUGAIN.pdf
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https://hal.univ-lorraine.fr/tel-01746945
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Submitted on : Thursday, March 29, 2018 - 10:48:13 AM
Last modification on : Monday, August 31, 2020 - 3:52:19 PM

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  • HAL Id : tel-01746945, version 1

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Estelle Maugain. Etude des modalités de mort cellulaire suite à un traitement photodynamique avec le Foscan® in vitro. Relation entre la distribution intratumorale du Foscan® et l'efficacité de la thérapie photodynamique in vivo chez la souris. Biotechnologies. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10249⟩. ⟨tel-01746945⟩

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