La culture de macrophages comme modèle prédictif de la toxicité et de la biopersistance des fibres minérales artificielles

Abstract : Toxicity of Man Made Mineral fibers (MMMF) is usually evaluated by biopersistence tests on laboratory animals. Toxicologists and manufacturers are looking for predictive in vitro methods in order to limit in vivo testings. Our aim was to perform an in vitro testing method in order to study MMMF persistence after contact with human cell line monocytes (U-937). Macrophage aclivation is an essential parameter to obtain a better MMMF in vitro degradation. With bacterial extracts as weil as synthetic cytokines, we mimic physiological activation of macrophages leading to physical and chemical degradation of MMMf. Macrophage transcriptome analysis, using ADN chips under our test conditions, enable us to assume that reactive oxygen species generated by oxidative stress would be responsible for this degradation. MMMF degradation obtained in such conditions after a 7-day incubation period, is identical to that observed after in vivo testing, and is well related to in vivo biopersistence experiments. Thus, we established an in vitro predictive test to allow preliminary screening of mineral fibres new compositions without the lise of any rodent.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_2005_0229_DIKA_NGUEA.pdf
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https://hal.univ-lorraine.fr/tel-01746990
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Submitted on : Thursday, March 29, 2018 - 10:49:06 AM
Last modification on : Monday, April 16, 2018 - 10:40:42 AM

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Hermine Dika Nguea. La culture de macrophages comme modèle prédictif de la toxicité et de la biopersistance des fibres minérales artificielles. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2005. Français. ⟨NNT : 2005NAN10229⟩. ⟨tel-01746990⟩

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