Cristallogenèse sous contraintes physiques : effet d'un champ électrique externe sur les solutions protéiques et leurs cristallisations

Abstract : This work deals with the solubility and crystallization properties of proteins and their behavior in solution under an external electric field. Our protein model is a hen egg-white lysozyme. The aim was to investigate how an external electric field can influence the nucleation, growth and solubility of lysozyme in solutions of various physico-chemical natures. The resulting diagrams have permitted to analyze the effect of the electric field on the solubility and the crystallization kinetics of lysozyme. This was used to propose a new ways to improve the crystal growth and new insights about how an external electric field can be rationally used to control effectively the protein crystal nuckleation. This result was confirmed by modeling calculations. Finally, our approach permits to obtain big crystals suitable for neutron diffraction experiments.
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Theses
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http://docnum.univ-lorraine.fr/prive/SCD_T_1999_0273_TALEB.pdf
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https://hal.univ-lorraine.fr/tel-01747105
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Submitted on : Thursday, March 29, 2018 - 10:51:06 AM
Last modification on : Monday, April 16, 2018 - 10:41:10 AM

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Majid Taleb. Cristallogenèse sous contraintes physiques : effet d'un champ électrique externe sur les solutions protéiques et leurs cristallisations. Autre. Université Henri Poincaré - Nancy 1, 1999. Français. ⟨NNT : 1999NAN10273⟩. ⟨tel-01747105⟩

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