Caractérisation de 69 bactériophages de Streptococcus salivarius subsp. thermophilus, incluant 10 bactériophages tempérés

Abstract : Twelve of the 118 strains of Streptococcus salivarius subsp. thermophilus of the CNRZ collection exhibited a Iytic phase after mitomycin C induction and contained active phages. The corresponding temperate phages were multiplied on indicator strains and compared to 56 phages isolated as Iytic. All Iysogenic strains were characterized by autolytic properties in certain conditions of culture. Morphologically, the 69 temperate or virulent phages studied all belonged to Bradley's (1967) group B, or to the family of the Siphoviridae of the International Committee on Taxonomy of Viruses (Matthews, 1982). There were 2 types of virion protein profiles. Most phage DNA were linear with cohesive ends, and size varied trom 35 to 50 kb. There was considerable restriction polymorphism. Homology existed among all phage DNA, in particular between the DNA of temperate phages and those of virulent phages. The study of phage-bacteria interactions showed that some Iysogenic strains were sensitive to superinfections by numerous phages and that in this case, these Iysogenic strains rearranged the genome of infecting phages. The results suggest that Iysogenic strains of S. salivarius subsp thermophilus could play a major role in the genesis of phage infections and in the evolution of the phage population specific to this streptococcus.
Document type :
Theses
Complete list of metadatas

Cited literature [127 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01747153
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 10:53:20 AM
Last modification on : Monday, April 16, 2018 - 10:41:51 AM
Long-term archiving on : Thursday, September 13, 2018 - 2:21:44 PM

File

SCD_T_1993_0011_FAYARD.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01747153, version 1

Collections

Citation

Blandine Fayard. Caractérisation de 69 bactériophages de Streptococcus salivarius subsp. thermophilus, incluant 10 bactériophages tempérés. Sciences agricoles. Université Henri Poincaré - Nancy 1, 1993. Français. ⟨NNT : 1993NAN10011⟩. ⟨tel-01747153⟩

Share

Metrics

Record views

21

Files downloads

13