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Réponse moléculaire, physiologique et génétique du pin maritime à une contrainte hydrique

Abstract : The main goal of this study was to increase our knowledge regarding the response of maritime pine (Pinus pinaster Aït) seedlings faced to a progressive water deprivation. A multidisciplinary approach (molecular biology, genetics, physiology and biometrics) was developed. Two-years-old seedlings belonging to a F2 segregating family were used. A genetic map covering about 94% of the genome was first constructed with AFLP, RAPD and proteins markers. This map allowed to locate the loci controlling quantitative variations (QTL : quantitative trait loci) of physiological traits. Two-dimensional gel electrophoresis allowed to anaIyse the protein pattern of needles in two conditions contrasting for water availability. Forty-five needle proteins were characterised by micro-sequencing. The combined information of protein functions, variation of protein amounts between non-stressed and stressed conditions, homology of protein expressions, protein pattern differences between genotypes, suggested that drought stress' provoked profound alterations in cellular metabolism. Furthermore, detection of PQL (protein quantity loci) showed that different genomic regions were involved in a complex regulation of those alterations. Even if we can not propose selection parameters for drought resistance, the analysis of PQL and QTL showed that three different genomic regions are involved in water deficit response of maritime pine seedlings.
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Submitted on : Thursday, March 29, 2018 - 10:56:02 AM
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Paulo Jorge Marques da Costa. Réponse moléculaire, physiologique et génétique du pin maritime à une contrainte hydrique. Sylviculture, foresterie. Université Henri Poincaré - Nancy 1, 1999. Français. ⟨NNT : 1999NAN10297⟩. ⟨tel-01747265⟩



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