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Etude de l'infection à Helicobacter pylori et de la pénétration de l'amoxicilline dans des estomacs d'embryons humains greffés chez des souris Nude

Abstract : Different in vitro or animal models have been used to investigate the pathogenesis of Helicobacter pylori infection and the gastric penetration of antibiotics used to eradicate this microorganism. However, extrapolation to humans of results obtained with these heterologous models remains difficult. We have developed a new model for the study of H. pylori infection that uses human embryonic stomachs engrafted in «nude» mice. At 80 days after implantation, these organs, which exhibited a well differentiated epithelium, were inoculated with a freshly isolated H. pylori isolated strain (H. pylori LB1) or with a reference strain (H. pylori ATCC 43504 or H. pylori ATCC 49504). After 12-week examination, only H. pylori LB1 persistently colonized the antral mucosa. Intracytoplasmic location of this microorganism was also obseNed in the antrum or the fundus. Then, we have compared the penetration of amoxicillin, after intraperitoneal administration, in the gastric mucosa of infected (H. pylori LB1) or uninfected grafts. Serum concentrations were similar to those obtained in humans after oral administration of 1 g of amoxicillin. Tissular concentrations were significantly higher in infected grafts. Thus, this model should allow to study the interaction between H. pylori and human gastric mucosa, and the penetration of xenobiotics in the infected mucosa.
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http://docnum.univ-lorraine.fr/prive/SCD_T_1999_0322_LOZNIEWSKI.pdf
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https://hal.univ-lorraine.fr/tel-01747267
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Submitted on : Thursday, March 29, 2018 - 10:56:12 AM
Last modification on : Thursday, January 21, 2021 - 3:01:01 PM

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  • HAL Id : tel-01747267, version 1

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Alain Lozniewski. Etude de l'infection à Helicobacter pylori et de la pénétration de l'amoxicilline dans des estomacs d'embryons humains greffés chez des souris Nude. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 1999. Français. ⟨NNT : 1999NAN19910⟩. ⟨tel-01747267⟩

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