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Orthobunyavirus de la République Centrafricaine : détection, séquençage et analyse phylogénétique

Abstract : The Orthobunyavirus genus is composed of segmented negative sense RNA viruses that are responsible for mild to severe human diseases. To date, no molecular studies of Bunyaviridae of the genus Orthobunyavirus from Central Africa have been reported, and their classification relies on serological testing. We have designed and evaluated four new primer pairs for amplification by RT -PCR and sequencing of the complete genomic small (S) RNA segments often Orthobunyavirus viruses isolated from Central African Republic (CAR) and pertaining to 5 different serogroups. Phylogenetic analysis showed that these 10 viroses belong to the Bunyamwera serogroup. The S segment sequences differ from those of the Bunyamwera virus reference strain by 5-15 % at the nucleotide level, and both overlapping reading frames encoding nucleocapsid (N) and non-structural (NS) protein were evident in sequenced genomes. Partial sequencing of M segment was also performed and inversely to the S fragment, the obtained phylogenetic p-ee was not monophyletic as M'Poko strain was highly divergent. This study should improve diagnosis and surveillance of African bunyaviruses.
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Emmanuel Nakouné-Yandoko. Orthobunyavirus de la République Centrafricaine : détection, séquençage et analyse phylogénétique. Biologie moléculaire. Université Henri Poincaré - Nancy 1, 2007. Français. ⟨NNT : 2007NAN11306⟩. ⟨tel-01747446⟩

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