Etude de la régulation de l'expression des gènes chez Escherichia coli, en lien avec le transport et le métabolisme du glucose : comprenant : - l'analyse moléculaire de la région promoteur dirigeant l'expression des gènes gapA et yeaA, - l'étude de l'effet de l'absence de protéine EIIBCGlc assurant le transport spécifique du glucose sur le transcriptome d'Escherichia coli.

Abstract : In Escherichia coli, the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a key enzyme for glucose metabolism and is encoded by the gapA gene. The transcription of this gene is initiated at 4 promoters and mainly at the gapA P1 promoter in the exponential phase of growth. We found that altought beeing a promoter of the -10 extended class, promoter P1 needs a -35 hexamer for activity. The E. coli methionine sulfoxide reductase of the B type (MsrB) is encoded by the yeaA gene which is located upstream and in the opposite direction of gapA. We showed that elements of the gapA-yeaA inter-ORF sequence are involved in the regulation of the two genes, either activating both genes or having opposite effects on them and we obtained data in favor of a translation regulation by the small non-coding RNA RyhB of the MsrB protein expression. We developped a DNA macroarray approach to test for the effect of the inactivation of the EIIBCGlc protein on the E. coli transcriptome. We showed that the expression of genes allowing formation of large pores in the external membrane and flagella were upregulated. We demonstrated the increased motility of the bacteria.
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Benoit Thouvenot. Etude de la régulation de l'expression des gènes chez Escherichia coli, en lien avec le transport et le métabolisme du glucose : comprenant : - l'analyse moléculaire de la région promoteur dirigeant l'expression des gènes gapA et yeaA, - l'étude de l'effet de l'absence de protéine EIIBCGlc assurant le transport spécifique du glucose sur le transcriptome d'Escherichia coli.. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10191⟩. ⟨tel-01748108⟩

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