Contribution à l'étude de la structure et du polymorphisme du génome du basidiomycète ectomycorhizien "Laccaria bicolor" (Maire) Orton et identification de QTLs de mycorhization chez les peupliers, "Populus trichocarpa Torr. & A. Gray ex Hook. et "Populus deltoides (Bartr.) Marsh

Abstract : The mycorrhizal symbioses between fungi and roots concern 95 % of the plant species. Social trees of boreal and temperate forests form a particular type of root association with fungi: the ectomycorrhizal symbiosis. Ectomycorrhizas play a major role in tree hydromineral nutrition, nutrient cycles and primary production. However, their complexity have so far prevented from deciphering their precise function and role. The recent availability of the genome of the ectomycorrhizal fungus Laccaria bicolor and that of the host-tree Populus trichocarpa provides an unprecedented opportunity to decipher the key components of development and functioning of this symbiosis. The aims of this study were to participate to the characterization and deciphering of the genome of L. bicolor, and to determine the genes involved in the formation of ectomycorrhizas in both partners. Firstly, in order to facilitate the assembly of the genomic sequence of L. bicolor, we have identified the repeated sequences and generated a genetic map. On the 60 Mb of this genome, 8 % are microsatellite sequences and 24 % transposable elements. A genetic map was built from 111 monokaryons issued from L. bicolor S238N. This map includes 326 markers (8 RAPD, 243 AFLP, 59 SSR and 14 SNP) distributed on 10 linkage groups anchored onto the genomic sequence of L. bicolor. Secondly, we have identified the genes involved in the establishment of ectomycorrhizas in poplar by combining QTL detection and DNA microarrays. We targeted 81 genes which can be involved in the establishment and/or the functioning of the symbiosis.
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Jessy Labbé. Contribution à l'étude de la structure et du polymorphisme du génome du basidiomycète ectomycorhizien "Laccaria bicolor" (Maire) Orton et identification de QTLs de mycorhization chez les peupliers, "Populus trichocarpa Torr. & A. Gray ex Hook. et "Populus deltoides (Bartr.) Marsh. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2009. Français. ⟨NNT : 2009NAN10079⟩. ⟨tel-01748313⟩

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