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Influence des conditions de culture sur la différenciation de progéniteurs vasculaires en vue de l'obtention d'un substitut vasculaire autologue

Abstract : The increase of vascular pathologies is going to create these next years, important needs in vascular substitutes of small calibres. The gold standard, this day, remains the use of autologous vessels reducing the infectious and immunological risks, but of limited custom, which is at the origin of tissue engineering. The identification of vascular progenitor cells is of major interest in vascular engineering. These autologous cells present a high capacity of proliferation and a potential of differentiation in vascular cells (endothelial cells (EC) and smooth muscle cells (SMC)) but the difficulty lies in the choice of the coated surface of culture which facilitates their adhesion and proliferation. In this work, we chose polyelectrolytes multilayer films and measured their impact on these vascular progenitor cells in various conditions of culture (normoxia or hypoxia). We showed at first, that these polyelectrolytes multilayer films allowed to accelerate the differentiation of these cells into matures EC. We also showed that these same cells cultivated under hypoxic conditions were able to differentiate into stable and contractile cells, presenting a phenotype comparable to the mature SMC. The association of these results added to the advantages brought by detachable sheet is the basis of the construction of an autologous small calibre vascular substitute consisting of EC and SMC differentiated from the same pool of cells, but cultivated in different conditions.
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https://hal.univ-lorraine.fr/tel-01748336
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Submitted on : Thursday, March 29, 2018 - 11:32:04 AM
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  • HAL Id : tel-01748336, version 1

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Nicolas Berthélémy. Influence des conditions de culture sur la différenciation de progéniteurs vasculaires en vue de l'obtention d'un substitut vasculaire autologue. Biologie cellulaire. Université Henri Poincaré - Nancy 1, 2009. Français. ⟨NNT : 2009NAN10085⟩. ⟨tel-01748336⟩

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