Contribution à l'étude des transporteurs de phosphate de la famille PHT1 chez le Peuplier (Populus trichocarpa Torr. & Gray) et le champignon ectomycorhizien Laccaria bicolor (Maire) P. D. Orton

Abstract : The publication of the first genome sequence of a tree - the poplar - and of an ectomycorrhizal fungus - Laccaria bicolor - has opened new prospects in forestry. Because their genomes are now known, and these species are important both economically and ecologically,Poplar and L. bicolor comprise an important model of plant : fungal interactions,. The mycorrhizal symbiosis, occurring between a fungus and a plant, improves plant mineral nutrition, especially for phosphorus. Forest management requires a better understanding of the mechanisms utilized by plants growing in low phosphorus soils. To increase our understanding of these processes, we characterized the multigenic families of Pi transporters (PHT1 type) of both organisms. We elucidated the subtle regulation of these transporters, from both partners, in various physiological states. Plants grew faster when mycorrhizal and this effect might be explained by the regulation of certain of the Pi transporters : LbPht1;5 in L.bicolor; PtrPht1;9 and PtrPht1;12 in poplar associated with L.bicolor, and PtrPht1;10 associated with G.intraradices. Other Pi transporters responded specifically to stimuli such as leaf senescence (PtrPht1;1, PtrPht1;5, PtrPht1;6, PtrPht1;9 and PtrPht1;12) or Pi starvation (PtrPht1;9, PtrPht1;12 in poplar, LbPht1;4 in L. bicolor). In addition, we observed different levels of expression of each Pi transporter in different tissues of each partner. This mosaic of responses of PHT1 family members provides an initial understanding of how mycorrhization affects Pi uptake from the fungus and plant. From this solid base of knowledge, the mechanisms by which each partner takes up Pi, either directly from soil, or indirectly via their symbiont, can be further expored.
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Marline Véronica Pereda Campos. Contribution à l'étude des transporteurs de phosphate de la famille PHT1 chez le Peuplier (Populus trichocarpa Torr. & Gray) et le champignon ectomycorhizien Laccaria bicolor (Maire) P. D. Orton. Sciences agricoles. Université Henri Poincaré - Nancy 1, 2008. Français. ⟨NNT : 2008NAN10141⟩. ⟨tel-01748378⟩

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