Microspectromètrie d'impédance basses fréquences : application à la mesure de milieux biologiques

Abstract : We present a low frequency approach to characterize small biological samples by using bioimpedance spectroscopy. The state of the art of existing approaches and technologies for the determination of electrical parameters of single cell or aggregate of cells is developed in the two first chapters. An electronic instrumentation allowing to characterize an isolated Xenopus ovocyte cell in the frequency range 50 Hz - 5MHz is presented in the third chapter. This consists of an impedancemeter and a biosensor based on two coplanar gold measurement electrodes. The theoretical validation of the chip uses an electrical modeling of the sensing area by finite elements method. A second approach is then proposed in the fourth chapter for the characterization of small biological fluidic samples. It uses a set of 4 coplanar platinium microband electrodes combined with an electronic conditioning circuit designed to operate in the 1 KHz - 100 kHz frequency range. As it is well known, the use of a tetrapolar configuration for the electrodes system prevents the influence of the polarization impedance at the metal-medium interfaces. The sensing area is also numerically modeled by finite elements method allowing to calculate the cell factor. This parameter was compared with its values deduced experimentally. The microspectrometer designed was thus experimentally validated. Both calibrations and results for the characterization of 50 µl human blood samples are presented and discussed.
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Cédric Margo. Microspectromètrie d'impédance basses fréquences : application à la mesure de milieux biologiques. Autre. Université Henri Poincaré - Nancy 1, 2009. Français. ⟨NNT : 2009NAN10051⟩. ⟨tel-01748395⟩

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