Etude de l'allergénicité de la caséine bovine : réponse proliférative des lymphocytes en mastocytes à l'alimentation en caséine et mise en évidence de l'absorption intestinale des antigènes chez des souris

Abstract : Feeding 100 mg/day of casein in mice resulted in in vitro lymphoblastic responses in the presence of the anttgen and total IgG production in the plasma as well as in the culture supernatants. This sensibilization was evaluated by lymphoblastic transformation test (LIT) where the presence of casetn in the culture medium stimulated lymphocytes 3H-thymidine incorporation. Casein ingestion at younger age (before 2 months) sensitized the mice more rapidly and intensively. Lymphocytes sensitized by casein ingestion recogntzed in vitro not only casein but its digestive enzyme hydrolysed fragments, which signifled that the hydrolysts processes by the three enzymes did not eliminate the antigents determinant of casein. Plasma and cultured lymphocyte total IgG productions were not accompanied by the production of specific IgG anti casein. In addition, there was no indication of IgE production in the fed mice, as was shown by the negative results of mastocyte histamine antigenic liberation test and cutaneous test. However, mastocyte histamine accumulation was observed in the fed mice. Since lymphocyte sensttization and mastocyte histamine accumulation caused by casein ingestion are important factors in allergie reactions, the results of this study should be considered in casein feeding practice. The results of intestinal absorption experiments using mouse everted intestinal sacs revealed the passage of antigens issued from casein and its peptic (P) and peptic-tryptic-chymotryptic (PTC) hydrolysates. Further analysis by electrophoresis and autoradiography showed only the absorption of fragments derived from P hydrolysate, which had a molecular wetght around 2500 Da. However, identification by ultrafiltration method showed the absorption of peptides issued from casein and its hydrolysates having the molecular welght inferior to 3000 Da. These absorbed fragments sttll contained the anttgentc sites, as demonstrated by the results of the immunodotting and radioimmunodotting tests. The anttgentcity of casein and its hydrolysate HPLC fractions were determined by ELISA and immunodotting method ...
Document type :
Theses
Complete list of metadatas

https://hal.univ-lorraine.fr/tel-01748585
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 11:42:06 AM
Last modification on : Friday, July 13, 2018 - 4:42:03 PM
Long-term archiving on : Friday, September 14, 2018 - 9:58:14 AM

File

SCD_T_1991_0110_RUNGKAT_ZAKARI...
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01748585, version 1

Collections

Citation

Fransiska Rungkat-Zakaria. Etude de l'allergénicité de la caséine bovine : réponse proliférative des lymphocytes en mastocytes à l'alimentation en caséine et mise en évidence de l'absorption intestinale des antigènes chez des souris. Biochimie, Biologie Moléculaire. Université Henri Poincaré - Nancy 1, 1991. Français. ⟨NNT : 1991NAN10110⟩. ⟨tel-01748585⟩

Share

Metrics

Record views

19

Files downloads

173