Développement de marqueurs moléculaires chez le pin maritime (Pinus pinaster Ait.) et cartographie génétique comparée des conifères

Abstract : Maritime pine (Pinus pinaster) is an economically and ecologically important forest tree species in southwestern Europe. A breeding program was started by INRA in the 60's and was recently enhanced by the toolkits of genomics. The objectives of the present work were twofold: 1/ construct a saturated genetic linkage map for maritime pine and 2/ compare this map to that of other conifers. Molecular and in silico approaches were used to develop molecular markers. A total of 766 AFLPs, 54 ESTs, 30 SSRs and 9 SNPs were mapped. While AFLP markers made it possible to quickly saturate the linkage map, ESTs and SSRs were used as orthologous markers to align the P. taeda and P. pinaster maps. Homologous linkage groups between the two species were identified. Together with literature data our results indicate that the genome structure of conifer species did not profoundly change since their ancient divergence time. As an application to this study, QTL and candidate genes for wood quality in pines were verified.
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David Chagné. Développement de marqueurs moléculaires chez le pin maritime (Pinus pinaster Ait.) et cartographie génétique comparée des conifères. Biologie végétale. Université Henri Poincaré - Nancy 1, 2004. Français. ⟨NNT : 2004NAN10008⟩. ⟨tel-01748676⟩

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