Surveillance et contrôle de la rougeole : caractérisation du virus et de l'immunité humorale

Abstract : Molecular epidemiology of measles virus as weil as the evaluation of humoral immunity have proven to facilitate measles surveillance and control. We studied the evolution of humoral immunity against measles after vaccination and after convalescence in longitudinal studies. Accelerated antibody decay rates were found among vaccinees with high antibody levels at the beginning of the study period, whereas low antibody levels were relatively stable. Re-exposure to wild-type virus induced a longlasting stabilization of antibody levels in convalescents. Since the proportion of vaccinated individuals is continously increasing and wild-type virus circulation is low, regular studies of humoral immunity will be crucial to assess the level of protection of in the corresponding populations. Antibody detection in oral f1uid has been promoted as an alternative to serum in the past. Our evaluation of a commercialised ELISA however shows a reduced sensitivity of detection compared to serum when antibody levels were low. ln order to facilitate measles virus genotyping on a large number of sam pies and in absence of sequencing facilities we have developed a simplified genotyping method to distinguish between actif measles virus clades and genotypes. This method is based on 6 different multiplex PCRs, which give PCR products of characteristic sizes for each clade or genotype. The method was developed using virus isolates of the different genotypes and evaluated and further optimized using clinical samples collected from measles patients.
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Jacques Kremer. Surveillance et contrôle de la rougeole : caractérisation du virus et de l'immunité humorale. Médecine humaine et pathologie. Université Henri Poincaré - Nancy 1, 2006. Français. ⟨NNT : 2006NAN11303⟩. ⟨tel-01748677⟩

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