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Utilisation de cellules souches médullaires en bioingénierie tissulaire du ligament

Abstract : Ligaments play an important role in the movement and stability of joints. Accidents and chronic fatigues are the main reasons for ligament lesion which usually is difficult for self healing and leads to serious dysfunction of ligaments and joints. The construction of bioengineering ligaments gives a new way to overcome this problem. Cells are very important in the construction of a biotissue with appropriate biological as well as mechanical properties. Which cellular source and microenvironment should be used for the reconstruction of ligaments? The objective of this work is to study the differentiation of bone marrow mesenchymal stem cell (BMSC) into fibroblast. We co-cultured indirectly rat BMSC with ligament fibroblasts or stimulated them by mechanical stretching. After that, the expressions of characteristics mRNA and protein of ligaments (collagen I, III and tenascin-C) have been analyzed. Our experimental results showed that the culture of BMSC in a microenvironment of ligament fibroblast or under stretching favored the syntheses of collagen I, III and tenascin-C in the proportions close to ligaments. In summary, these studies suggest it is feasible to use BMSC as cellular source for a clinical application in tissue engineering of ligaments
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https://hal.univ-lorraine.fr/tel-01748683
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Lei Zhang. Utilisation de cellules souches médullaires en bioingénierie tissulaire du ligament. Médecine humaine et pathologie. Institut National Polytechnique de Lorraine, 2008. Français. ⟨NNT : 2008INPL001N⟩. ⟨tel-01748683⟩

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