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Etude des mécanismes de structuration d'assemblages [bêta]-lactoglobuline-gomme d'Acacia en présence d'un flavonoïde, la quercétine

Abstract : Flavonoids are promising ingredients in food and non food applications through their antioxidative and biological properties. However, their negligible solubility in most solvent renders problematic technological developments. In order to overcome this drawback, the approach followed in this study was the incorporation of flavonoids in stable and functional particles based on biopolymers (protein-polysaccharide). The main objective of this thesis was to acquire knowledge at different scales to study the mechanisms of interaction and linkage between a flavonoid, quercetin, and two biopolymers,[bêta]-lactoglobulin (BLG) and Acacia gum (AG). In a first step, we were able to develop submicronic particles (Dh : ~250 nm) of quercetin-Acacia gum (GAQ) based on a core of quercetin and a shell of Acacia gum, reflecting a behavior of soft particles. In a second step, we investigated the assembly mechanisms GAQ with BLG at pH 4.2 and 25 ° C. The thermodynamic parameters of interaction were determined by isothermal titration calorimetry (ITC) and show binding isotherm characterized by an exothermic-endothermic sequence. The various structural entities formed during the complexation between GA and BLG or BLG and GAQ were characterized using the same approach as for the ITC titration. Finally, measurements using infra-red Fourier transform (FTIR) showed changes in the secondary structure of BLG after interaction with the GA and the GAQ. There was a significant loss of secondary structures (-helices and -sheets), even more pronounced in presence of quercetin
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Leïla Aberkane. Etude des mécanismes de structuration d'assemblages [bêta]-lactoglobuline-gomme d'Acacia en présence d'un flavonoïde, la quercétine. Autre. Institut National Polytechnique de Lorraine, 2010. Français. ⟨NNT : 2010INPL045N⟩. ⟨tel-01748834⟩

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