Etude des effets génotoxiques et de l'induction des phytochélatines chez Vicia faba (Fabaceae) exposée au cadmium : application du test Vicia micronoyaux à des matrices complexes

Abstract : Higher plants demonstrate defence mechanisms whereby they are able to respond to both nutrient deficiencies and toxicants. In this work, cadmium genotoxicity and quantitative relationshps between different hierarchical endpoints in Vicia faba (broad bean) cultivated in both contaminated environment (i.e. hydroponical and soils) were studied. Increases of miaonucleus (MN) frequencies (genotoxicity endpoint), phytochelatins induction and antioxidative enzyme activities (i.e. superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase, glutahone reductase) and manodialdehyde (MDA), indicator of lipid peroxidation, were analysed. To study the process of stress 1 adaptation in Vicia faba plants, different realistic CdCl2, concentrations were employed. f In hydroponic culture, the increase of micronucleus frequency was the first cadmium i , exposure effect that appeared (LOEC: 7,5 10-8 M CdCl2). Then decreases of antioxidative enzymes activities were observed (APX, SOD, CAT at 10-'M; MDA for 10-5M). Phytochelatins induction was detected from IO-~Mb, ut it was more effective from 10-'M. A tirne-concentration dependent response of cadmium was observed, for these cadmium exposure indicators. A tissue dependent response of cadmium was observed for enzymes activities and phytochelatins induction too. The micronuclei bioassay was also tested with V. faba plants directly exposed in cadmium polluted soils and on leachates from multi contaminated soils. Our results suggest that the synthesis of phytochelatins, observed in higher degrees of stress, was related to the inactivation of other tolerance mechanisms and that it was associated with the metabolic cost of these mechanisms. The results also show that V. faba micronuclei bioassay would be efficient for in situ studies of contaminated soils
Document type :
Theses
Complete list of metadatas

Cited literature [46 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01748975
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 11:56:35 AM
Last modification on : Monday, April 16, 2018 - 10:43:01 AM
Long-term archiving on : Friday, September 14, 2018 - 10:46:38 AM

File

Beraud.Eric.SMZ0700.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01748975, version 1

Collections

Citation

Eric Béraud. Etude des effets génotoxiques et de l'induction des phytochélatines chez Vicia faba (Fabaceae) exposée au cadmium : application du test Vicia micronoyaux à des matrices complexes. Sciences agricoles. Université Paul Verlaine - Metz, 2007. Français. ⟨NNT : 2007METZ013S⟩. ⟨tel-01748975⟩

Share

Metrics

Record views

31

Files downloads

15