Utilisation de biomarqueurs cellulaires chez plusieurs espèces d'invertébrés pour l'évaluation de la contamination des milieux duçaquicoles

Abstract : To validate a cellular biomarker suite, we studied the lysosomal and peroxisomal structures as well as the variations of the lipofuscin and unsaturated neutral lipid contents in the digestive tissues of several freshwater invertebrates, by using histochemistry coupled to image analysis. Cellular responses, studied through field and laboratory experiments proved relevant to highlight contamination gradients. The most frequent response profiles in molluscs were lysosomal enlargement, unsaturated neutral lipid depletions, lipofuscin accumulations and a more voluminous peroxisomal system. These early cellular responses may be associated, at the individual level, to a desynchronisation of the reproduction or to decreased condition index. A long-term monthly experiment showed no seasonal baseline levels of these biomarkers and underlined the important influence of trophic factors on the unsaturated neutral lipid contents. A 15-day depuration period of the bivalve, Dreissean polymorpha, at the site of origin after an exposure to a comparison of integrative analysis tools proved that the whole biomarker suite discriminates better between sites than individual responses
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Edwige Guerlet. Utilisation de biomarqueurs cellulaires chez plusieurs espèces d'invertébrés pour l'évaluation de la contamination des milieux duçaquicoles. Sciences agricoles. Université Paul Verlaine - Metz, 2007. Français. ⟨NNT : 2007METZ057S⟩. ⟨tel-01749087⟩

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