Réhabilitation de sols pollués par des HAP grâce aux bactéries associées à la rhizosphère de Miscanthus X giganteus

Abstract : The aim of this work was to investigate the potential of Miscanthus x giganteus (MxG) for PAH polluted soils remediation and fly ash revegetation. In vitro studies through microplate assays demonstrated the contribution of MxG root exudates and particularly of quercetin (following the identification of root secondary metabolites) to the selective biostimulation of PAH-degrading bacteria. To get insights into bioremediation processes and rhizosphere effects on soil quality, microcosm experiments were conducted at a laboratory scale, using artificially reconstituted soils and collected industrial soils. On the one hand, significant dissipations of four-ring PAH were noticed in the rhizosphere of contaminated soils. On the other hand, significant organic carbon and nitrite inputs could be measured in fly ash, indicating a "restart" of nitrogen cycle and suggesting an enhancement of fly ash weathering processes in the long term. Besides, the development of a soil nucleic acid extraction and purification method permitted to study the evolution of bacterial community diversity in all types of soils, based on PCR-TTGE of eubacterial 16S rDNA. Specific enrichments of bacterial phylotypes could be reported in rhizoplanes, confirming root-associated processes on the selection of soil bacterial communities. Finally, plant adaptation to the broad range of studied substrates in situ lead us to suggest that MxG cultivation could be an effective strategy for a new system of sustainable agricultural activities in wasteland soils
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Didier Técher. Réhabilitation de sols pollués par des HAP grâce aux bactéries associées à la rhizosphère de Miscanthus X giganteus. Sciences agricoles. Université Paul Verlaine - Metz, 2011. Français. ⟨NNT : 2011METZ047S⟩. ⟨tel-01749177⟩

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