Evaluation de l'état de viabilité et du pouvoir d'infectiosité de trois micro-organismes pathogènes pour l'homme (bactérie : Campylobacter, virus : Adenovirus et parasite : Cryptosporidium) détectés dans des échantillons d'eaux destinées à des fins alimentaires

Abstract : The aim of this study was (i) to determine the occurrence and (ii) to study the behavior of different microorganisms known to cause human waterborne outbreaks in raw waters (surface water and groundwater) and treated water of several reservoirs along the Moselle river. Three pathogens were studied: bacteria (C. jejuni and C. coli), enteric viruses (human adenoviruses which enteric adenoviruses), parasites (C. parvum, C. hominis, C. meleagridis). Because of these micro-organisms have different properties such as size, various methodologies were used for their detection, quantification and viability and/or infectivity. In the study of occurrence we showed that the Moselle river was heavily contaminated by these pathogens in varying concentrations depending on time of the year. For example it was found bacteria C. jejuni more frequently in summer than during winter while the reverse was observed for human adenoviruses. The results obtained in groundwater showed that despite a protection linked to the natural filtration of water, they could be contaminated with viable campylobacters and enteric adenoviruses. In the treated water, several genomic signatures associated with these two agents (bacterial and viral) were revealed by molecular biology tools without any risk related to the viability and infectivity can be established. In these waters, the inactivation experiments clearly showed the high sensitivity of these two agents to chlorination but not the parasite Cryptosporidium, which known to be the most resistant pathogens, whatever the type of water or incubation temperature
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Adeline Tissier. Evaluation de l'état de viabilité et du pouvoir d'infectiosité de trois micro-organismes pathogènes pour l'homme (bactérie : Campylobacter, virus : Adenovirus et parasite : Cryptosporidium) détectés dans des échantillons d'eaux destinées à des fins alimentaires. Autre. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0034⟩. ⟨tel-01749204⟩

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