Développement et validation de microbiotests en phase solide pour l'évaluation écotoxicologique de sédiments

Abstract : The sediments are a compartment of the aquatic environment that tends to accumulate pollutants through the deposition of suspended solids. Sediment contamination can have potentially negative effects on aquatic organisms, so it is necessary to monitor, assess and protect the sediments to ensure the integrity of the structure and functioning of aquatic ecosystems. The ANR-PRECODD DIESE (diagnostic tools for assessing sediment ecotoxicity) has joined efforts of different partners to develop a strategy for tiered environmental risk assessment of contaminated freshwater sediments. In the framework of this project our research was focused on the development and optimization of solid phase microbiotests to assess the ecotoxicity of freshwater sediments. The microbiotests selected in this study were the tests Microtox® solid phase (MSPA), the algal solid phase test, the test LuminoTox solid phase (Lum-SPA) and Ostracodtoxkit®. These biotests are characterized by different test conditions and assessment criteria. We have obtained different responses for each tested sediment. The algal test was the only ones that have not reported sensitivities with all samples tested. Furthermore, we demonstrated that the response of the test Lum-SPA and MSPA were correlated with some geochemical characteristics of sediments. Finally, we synthesized the responses of three microbiotests using an index of integration allowing to discriminate between the observed sediment ecotoxicity
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Karen Burga Pérez. Développement et validation de microbiotests en phase solide pour l'évaluation écotoxicologique de sédiments. Autre. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0219⟩. ⟨tel-01749312⟩

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