Effets d'expositions métalliques simples ou multiples à différentes températures chez Gammarus pulex : approche multi-marqueurs et perspectives en bioévaluation

Abstract : Multiple metal and metalloid contaminations impair aquatic ecosystem community structure and functioning. However, the mechanistic understanding of individual pollutant toxic effects, but also of their interaction effects in a "global warning" context are still rarely tackled. However, aquatic ecosystem managers have an urgent need of early warning indicators (i.e. early, sub-lethal) of toxicant effects. Recently, an increasing attention has been turned to studies of the proteome of species/populations, which is considered as a dynamic entity modified by environmental conditions. In this context, we have worked with a model organism, Gammarus pulex (crustacea, amphipoda), under laboratory controlled conditions. Three objectives have been defined: (1) assessing the single and combined effects of two metals (i.e. arsenate and/or cadmium), (2) understanding the potential confounding effect of temperature on measured physiological and behavioural responses of gammarids and (3) investigating the existence of a singular response of the proteome of individuals exposed to different single or combined metal concentrations, at several tested temperatures. The major goals were first applying a diagnostic analysis to provide valuable biomarkers early indicating metallic stress, and second developing the foundations for a prognostic approach better predicting the organismal consequences of toxic contaminations due to the anthropogenic degradation of aquatic ecosystems
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Céline Vellinger. Effets d'expositions métalliques simples ou multiples à différentes températures chez Gammarus pulex : approche multi-marqueurs et perspectives en bioévaluation. Autre. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0287⟩. ⟨tel-01749400⟩

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