Etude du développement de biofilms dans des réacteurs de traitement d'eau

Abstract : The development of biofilm has been studied over long periods of time (two to seven months) in laboratory-scale rotating biological contactors and fixed bed reactors continuously fed with municipal wastewater or synthetic growth media. Two reactors have been specifically designed for this purpose. The biofilms have been subject to hydrodynamic and chemical (antibiotics) stresses. The overall biological activity of the reactors have been monitored, in terms of carbon and nitrogen removal. The phenomena of sloughing and re-growth have been characterized on smooth and rough surfaces using image analysis non-destructive techniques. The amount of biomass present on the substratum has been evaluated by the biofilm opacity and this monitoring method has been validated by comparison with destructive methods such as crystal violet staining and dry weight. The biofilm macrostructure, related to growth, sloughing and re-growth phenomena, has been evaluated through visual texture characterization of the scanning gray level co-occurrence matrix (SGLDM) and the gray level run length method (GLRLM). The results shows the efficiency of image analysis as a rapid and cheap method to monitor biofilm development on the long term.
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Muatasem Alnnasouri. Etude du développement de biofilms dans des réacteurs de traitement d'eau. Autre. Institut National Polytechnique de Lorraine, 2010. Français. ⟨NNT : 2010INPL085N⟩. ⟨tel-01749431⟩

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