Transporteurs fongiques de manganèse : diversité et analyse fonctionnelle chez le champignon saprophyte Phanerochaete chrysosporium

Abstract : P. chrysosporium is a saprophytic fungus able to degrade many xenobiotics which makes it particularly attractive for applications in bioremediation. Several publications highlight the importance of metal homeostasis in the production of lignolytics enzymes. Indeed the presence of manganese is required for the production of manganese peroxidase. Conversely, deficiency allows the production of lignins peroxidases. Characterization of transporters involved in the control of manganese homeostasis has been only researched in the model S. cerevisiae. Analysis of putative manganese transporters of 26 fungal species representing 20 orders of fungus was used to form a repertory of 281 transporters of manganese. Phylogenetic analysis allowed to highlight that duplication process, but also deletion, had occurred particularly in S. cerevisiae. However this one is devoid of transporters belonging to the manganese Cation Diffusion Facilitator. Eleven transporters belonging to gene families in which manganese transporters have been found were identified in the P. chrysosporium?s genome. Expression level of these genes was examined particularly in ligninolytic condition. Transporters have also been cloned in order to verify their functions by complementation in heterologous system. This study allowed to identify putative manganese transporters of numerous fungal organisms and the lack of a transporters family involved in the manganese transport in S. cerevisiae
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Loïc Diss. Transporteurs fongiques de manganèse : diversité et analyse fonctionnelle chez le champignon saprophyte Phanerochaete chrysosporium. Sciences agricoles. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0189⟩. ⟨tel-01749606⟩

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