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Aldéhyde déshydrogénases non phosphorylantes : importance de la dynamique structurale au cours de la catalyse

Abstract : An essential feature of the catalytic mechanism of ALDH is the importance of flexibility and conformational dynamics in the active site, including the side chains of residues, substrate and cofactor. My thesis has identified bases responsible for the recognition of retinal controlling biosynthesis of retinoic acid in RALDH. I was then able to follow the flip of the cofactor reduced by using FRET, which allowed me to develop a kinetic model to determine the rate constant associated. Finally, the results obtained to identify the molecular basis responsible for late or early dissociation of the reduced cofactor showed that the mode of stabilization of the cofactor is the origin of this difference between these two families of enzymes
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https://hal.univ-lorraine.fr/tel-01749637
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Raphaël Bchini. Aldéhyde déshydrogénases non phosphorylantes : importance de la dynamique structurale au cours de la catalyse. Médecine humaine et pathologie. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0254⟩. ⟨tel-01749637⟩

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