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Étude des polymorphismes génétiques des patients porteurs de fentes labio-maxillo-palatines en lien avec le métabolisme des folates

Abstract : Background : Monocarbons determinants are involved in the etiology of cleft lip with or without cleft palate, based on ethnical background and periconceptional mother's diet. Genetic polymorphisms of actors of folate and monocarbon availability (MTHFR, MTR, NOS3, SHMT1 and 2, MTHFD1 and 2, MTHFS, SLC19A1, ABCB1, FOLR2 and FOLH1) are in association with the primary risk of oro-facial cleft in contrasting ways in Hispanic, non-Hispanic-white, Irish, Norwegian or Polish subjects. The actors of B12 availability like TCN2 are only involved in Italian subjects. Cholin metabolism (BHMT, BHMT2 and PCYT1A) is reported in Polan. CBS, actor of transulfuration pathway?s, is in association with cleft risk in Italian studies. Aim : This study is built to determine the primary risk of cleft (OCF) in association with single nucleotid polymorphisms of the actors of folate, B12 and choline availability, monocarbons metabolism and transulfuration pathway?s in France. Methods : We performed a case-control study of 250 children and their mothers (200), and 521 matches controls. A multiplex analysis of 384 SNPs (77 genes), selected by NCBI databases research's and in silico studies was performed, using GoldenGate Illumina technology (BeadExpress Reader, San Diego, California) with appropriate genotyping controls. Genotypes with call rate under 0.95 and SNPs with Hardy-Weinberg disequilibrium linkage were excluded. SNP association analyses were performed applying additive, dominant and recessive models to the data. Results :In univariate analysis of mothers versus controls for additive model 9 SNPs were found in association with primary risk of cleft, concerning rs2124459 CBS ; rs35789560 FPGS ; rs234709 CBS ; rs4816 PCMT1 ; rs737865 COMT ; rs4820889 TCN2 ;rs13043752 AHCY ; rs2168781 SHMT1 ; rs1801229 CUBN. After multiple testing correction, only CBS rs2124459 1552+1199A-->G reached statistical significance in additive models, with P= 0.009 in the additive model (OR (Dd) versus (dd) = 0.46 (0.26 to 0.81) and (DD) versus (Dd) = 0.51(0.26 to 0.97)). P= 0.09 in the dominant model (OR (DD, Dd) versus (dd) = 0.40 (0.23 to 0.69), respectively. In patients, CBS rs2124459 was significantly associated with a reduced risk of OFC in both additive and dominant models, with respectively P=0.026 and P=0.011. Discussion and conclusion : This study reported an intronic SNP from CBS not previously described, with a strong association with primary risk of cleft in mothers and child. These results argues to the predominant way of transulfuration in etiology of oro-facial cleft in France
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Submitted on : Thursday, March 29, 2018 - 12:22:31 PM
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Laetitia Goffinet. Étude des polymorphismes génétiques des patients porteurs de fentes labio-maxillo-palatines en lien avec le métabolisme des folates. Médecine humaine et pathologie. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0318⟩. ⟨tel-01749659⟩



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