Caractérisation de la réponse de deux cultivars de Vigna unguiculata à une contrainte ozone combinée ou non à la sécheresse : Régulation de protéines membranaires (AOX, PTOX et pUCP)

Abstract : Possible interactions between chloroplast and mitochondria remain poorly known, particularly in response to stress conditions. Under these conditions, it is suggested that the electron transfer pathways linked to AOX or pUCP (mitochondrial uncoupling proteins) and PTOX (plastidial uncoupling protein) could limit the formation of ROS to reduce oxidative damage in cellular organelles. In our work, we selected two cultivars of Vigna unguiculata, cv 1183 and cv EPACE. Under our experimental conditions, both cultivars showed no real differences in sensitivity to drought. However cv EPACE was more tolerant to O3 based on the development of necrosis and several physiological parameters (Fv/Fm, [phi]PSII) and biochemical (glutathione content). For the expression profiles of genes encoding AOX, PUCP and PTOX two responses were clearly identified in the cultivar EPACE. On a shortterm, expression of these proteins is generally stimulated. On a longer term (14 days), the answer differs depending on the treatment. Under O3, the strongest expression of mitochondrial proteins is maintained while the gene encoding the PTOX is down-regulated. Under drought only the plastid protein (PTOX) is upregulated. Under conditions of stress combination, drought has little effect on the influx of O3 in the leaves, and the VuPTOX and VuUCP1b genes are up-regulated after 3 and 7 days of stress. This over-regulation, already observed in response to drought alone could play a role in preventing the formation of ROS in both mitochondria and chloroplasts
Document type :
Theses
Complete list of metadatas

Cited literature [211 references]  Display  Hide  Download

https://hal.univ-lorraine.fr/tel-01749888
Contributor : Thèses Ul <>
Submitted on : Thursday, March 29, 2018 - 12:31:19 PM
Last modification on : Tuesday, July 23, 2019 - 4:00:35 PM
Long-term archiving on : Friday, September 14, 2018 - 10:39:35 AM

File

DDOC_T_2012_0258_MAIA_DE_SOUSA...
Files produced by the author(s)

Identifiers

  • HAL Id : tel-01749888, version 1

Collections

Citation

Francisco Yuri Maia de Sousa. Caractérisation de la réponse de deux cultivars de Vigna unguiculata à une contrainte ozone combinée ou non à la sécheresse : Régulation de protéines membranaires (AOX, PTOX et pUCP). Sciences agricoles. Université de Lorraine, 2012. Français. ⟨NNT : 2012LORR0258⟩. ⟨tel-01749888⟩

Share

Metrics

Record views

17

Files downloads

25