Les peptides de colza : une alternative aux protéines animales dans les procédés de culture de cellules de mammifères ?

Abstract : In order to improve the performances of animal cell culture processes for the production of therapeutic proteins, and to limit the risks of contamination by animal derived molecules, the effects of a supplementation of serum-free and animal-protein free culture media with rapeseed peptidic fractions were performed. Peptide fractions of various compositions were generated by enzymatic hydrolysis on proteic extract and membrane fractionation. These fractions, rich in nitrogen matter, displayed a variable effect on CHO cells, with a strong cell growth promoting effect of a fraction containing peptides with a broad range of size. In fact, this fraction increased the cell growth, prolonged cell survival, increased the specific production of the recombinant protein, and reduced the whole metabolism of carbohydrates. We also showed that tis peptidic fraction was utilized as nutritioinal additives, but also, as survival and/or growth promoting factors. Other additional advantages ot these peptides were highlighted, such as : an easier adaptation of various cell lines to serum-free conditions, a good cryoprotection of cells during the freezing process au good filterability. Indeed, a very simple culture medium was designed, completely free from animal molecules, and stimulating the growth of adherent or suspension cells from different industrial strains, for various culture scales. These results taken together suggest the use of this new particularly interesting peptidic source as an additive in animal cell culture processes.
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Bérangère Farges-Haddani. Les peptides de colza : une alternative aux protéines animales dans les procédés de culture de cellules de mammifères ?. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2005. Français. ⟨NNT : 2005INPL054N⟩. ⟨tel-01749982⟩

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