Détermination de conditions de mise en oeuvre d'une souche nouvellement isolée de Sporobolomyces ruberrimus pour la production de torularhodine

Abstract : Many microorganisms are able to synthesize carotenoids for industrial using. The aim of the present work is to study the production of an uncommon red pigment by a quasiunknown yeast growing in an inexpensive liquid medium. This study is divided mainly into three parts: the first one describes how a new strain of Sporobolomyces, SP. ruberrimus H11O, was isolated and identified. The second part is dealing with the breaking of the cell wall when using different chemical and physical methods. Among the methods tested, the crushing of the cells was the most efficient to recover the pigment by further ethanol extraction. But a low part of cellular poly-unsatured fatty acids are extracted too and needs to be separated. The carotenoids were identified as torularhodin and [bêta]-carotene with the aid of an analytical method specially developed using a H.P.L.C.-U.V. / A.P.C.I.-M.S. tandem and co-use F.T.I.R. In the last part, after the carbon source choice done, the kinetic parameters of the yeast growth and pigment production were determined when varying temperature or ammonium sulphate (nitrogen source) notably. Thus, technical glycerol is uptaken by the new strain to produce about 35 g dry cells L-1 and 3.7 mg torularhodin g-1 of cells that is to say 130 mg.L-1 in non-optimal culture conditions. One of the interests of this strain use is that torularhodin represents about 95 % of total carotenoids.
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Theses
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http://docnum.univ-lorraine.fr/prive/INPL_T_2004_RAZAVI_S_H.pdf
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https://hal.univ-lorraine.fr/tel-01750059
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Submitted on : Thursday, March 29, 2018 - 12:36:51 PM
Last modification on : Thursday, January 31, 2019 - 1:27:49 AM

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Seyed Hadi Razavi. Détermination de conditions de mise en oeuvre d'une souche nouvellement isolée de Sporobolomyces ruberrimus pour la production de torularhodine. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2004. Français. ⟨NNT : 2004INPL008N⟩. ⟨tel-01750059⟩

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