Étude cristallographique de glutathion transférases de micro-organismes impliqués dans la dégradation de la lignine : le basidiomycète Phanerochaete chrysosporium et la bactérie Sphingobium sp. SYK-6

Abstract : Phanerochaete chrysosporium is a very interesting saprophytic fungus because it decays wood, by degrading lignin while leaving cellulose which is a renewable source of energy. The soil bacterium Sphingobium sp. SYK-6 possesses enzymes (LigE, LigF and LigG) that cleave the beta-aryl ether linkage of lignin model compounds. LigE and LigF are glutathione dependent enzymes that reduce the ether bond (etherase activity) and LigG catalyzes the elimination of glutathione (lyase activity). This study presents the structure-function relationships of Lig enzymes and of two new classes of GST from P. chrysosporium : one potentially related to LigE (GSTFuA) and one potentially connected to LigG (GST Xi). The crystallographic structures of GSTFuA1, 2 and 3 from P. chrysosporium were solved. The analysis of the models reveals a new structural class of GST with unique properties. These characteristics could be connected to the ligandin function and to the catalytic pattern of this new class of GST. In parallel, structural studies of LigE and LigF from Sphingobium sp. SYK-6 are nearly completed. The crystallographic structure of the GSTX1 from P. chrysosporium exhibits specific structural properties which allowed us to define a new structural class (Xi) in the GST superfamily. The enzyme is a S-glutathionyl-(chloro)hydroquinone reductase (GHR) that does not present a lyase activity with LigG substrate. In parallel, high resolution structure of LigG was obtained; this enzyme can be related structurally and functionally to the GST Omega class
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Pascalita Prosper. Étude cristallographique de glutathion transférases de micro-organismes impliqués dans la dégradation de la lignine : le basidiomycète Phanerochaete chrysosporium et la bactérie Sphingobium sp. SYK-6. Autre [cond-mat.other]. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0155⟩. ⟨tel-01750222⟩

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