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Étude et modélisation de la cinétique de synthèse enzymatique de palmitates de glucose et de fructose

Abstract : The aim of this work is to study the kinetics of sugar esters synthesis in the presence of glucose or fructose and palmitic acid. These syntheses were conducted at 60°C in 2-methyl-2-butanol. Immobilised lipase B of Candida antarctica was used as biocatalyst. The explored parameters in this study are: the origin of lipase, the nature of the support, the type of immobilisation, the quantity of water and the solubility and dissolution rate of sugars. These studies showed that these reactions are affected mainly by the quantity of water, the solubility and the dissolution rate of sugars. The control of water by the use of molecular sieves allowed to reach a conversion yield of fatty acid about 98% for two sugars. However the reactions in the presence of glucose are slower compared to these conducted with fructose. This behaviour is due to the low solubility of the glucose (14 mM). The obtained results were structured in the form of a Bi-Bi ping pong kinetics model. This model was validated in a broad domain of concentrations of substrates and enzymes. It was used thereafter as a tool to define the best conditions of these reactions.
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Françoise Chamouleau. Étude et modélisation de la cinétique de synthèse enzymatique de palmitates de glucose et de fructose. Alimentation et Nutrition. Institut National Polytechnique de Lorraine, 2002. Français. ⟨NNT : 2002INPL120N⟩. ⟨tel-01750231⟩



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