Diversité fonctionelle des Glutation Transférases fongiques : caractérisation des classes Ure2p et GTT2 de Phanerochaete chrysosporium

Abstract : Phanerochaete chrysosporium is a forest fungus being part of saprophytic organisms able to recycle dead organic matter. Thanks to the excretion of numerous wood decaying enzymes, and especially lignin peroxidases, this fungus is able to break down plant material including lignin, a complex polymer of phenolic compounds. Lignin removal allows the release of other wood components such as cellulose and hemicellulose, which can be further used in paper industry or to produce second generation bioethanol. The structure of intermediates and products from lignin decomposition is close to that of numerous pollutants making P. chrysosporium biotechnologically interesting for bioremediation purposes. Moreover, the fungus has to deal with more or less toxic compounds created by degradation mechanisms. It thus presents a detoxification pathway involving enzymes including cytochrome P450 monooxygenases and glutathione transferases (GST). Ure2p enzymes belong to an extended GST class in Phanerochaete genus as well as in other saprophytic basidiomycetes. Their study based on phylogenetic, biochemical, structural and transcriptomic approaches provides a better understanding of evolution mechanisms of a class of enzymes potentially subject to strong selection selection pressure
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Anne Thuillier. Diversité fonctionelle des Glutation Transférases fongiques : caractérisation des classes Ure2p et GTT2 de Phanerochaete chrysosporium. Sciences agricoles. Université de Lorraine, 2013. Français. ⟨NNT : 2013LORR0219⟩. ⟨tel-01750515⟩

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